From the migration times measured for each fragment in the sample, the size is calculated. Two marker fragments for RNA only one marker fragment are run with each of the samples bracketing the overall sizing range. This is necessary to compensate for drift effects that may occur during the course of a chip run.
For DNA and protein assays, quantitation is done with the help of the upper marker. The area under the upper marker peak is compared with the sample peak areas. Because the concentration of the upper marker is known, the concentration for each sample can be calculated. Besides this relative quantitation, an absolute quantitation is available for protein assays, using external standard proteins.
For RNA assays, quantitation is done with the help of the ladder area. The area under the ladder is compared with the sum of the sample peak areas. The expert software plots fluorescence intensity versus migration time and produces an electropherogram for each sample. Adapted from the Agilent expert software online help. The main advantages of Lab-on-a-Chip are ease-of-use, speed of analysis, low sample and reagent consumption and high reproducibility due to standardization and automation.
Results are delivered within minutes in high quality digital data and can be shown in gel-like image, electropherogram and tabular formats. Skip to content — Skip to search. The University of Rhode Island. Each chip contains an interconnected set of microfluidic channels that are used for separation of the nucleic acid fragments by size using electrophoresis.
The fragments will move through the channels at different speeds dependent on their size and charge with smaller fragments moving more quickly than longer fragments. Please use ultra-pure water for any dilutions. Applications The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations.
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