Homogenization, also known as micronization or particle size reduction, is an important process step that is utilized in a variety of industries, including pharmaceutical, biotech, cosmetic, and food.
With benefits like improved taste, texture, and appearance, its no wonder that more and more industries are looking to purchase a homogenizer for their manufacturing needs!
One question that is frequently asked regards the capabilities of homogenizers versus a sonicator. If you are interested in learning more about sonication cell lysis and how it compares to homogenization, you are in the right place! Continue reading to learn more about sonication cell lysis, how it compares to homogenization, and what BEE International has to offer in the way of high quality, high pressure homogenization equipment.
Sonication is defined as the act of applying sound energy to agitate particles in a sample. Horizontal vs Vertical Laminar Flow Hoods. Horizontal vs.
Vertical Gel Electrophoresis Systems. How does an Ionizing Blow-Off Gun improve product quality? How to Clean a Laminar Flow Hood. How to Improve Pipetting Techniques. Incubator Features Comparison. Installing Flush-Mount Pass-Throughs. Installing Standard-Mount Pass-Throughs. Lab Balances and Scales Feature Comparison. Lab Transilluminator Features Comparison. Laboratory and Cleanroom Ovens Feature Comparison. Laboratory Evaporator Overview and Feature Comparison.
Laboratory Stirrers Feature Comparison Chart. Laboratory Waterbath Features Comparison. Laminar Flow Hoods Features Comparison.
Making Sense of Biological Safety Levels. Microarray Platforms: Beads, Slides or Chips? Moisture-Free Storage: Nitrogen or Desiccant? No Space for a Cleanroom? Operator Nausea from Work Surface Vibration. Pipette Features Comparison. Sonicators: How these agitating lab instruments work.
Spectrometry for Sample Analysis: Technique Overview. Static Control in Desiccator Cabinets. The Complete History of the Glovebox. The Importance of Airflow Uniformity.
Under Pressure in Critical Environments. Under the Microscope: What are Hoods? What are Anaerobic and Hypoxia Glove Boxes? What are the Benefits of a Nitrogen Generator? What is the best vacuum cleaner for my cleanroom? Because the energy is spread out, only a small amount of energy is imparted on each particle in the sample at a given time. Even in cases where they might work, the process would take a very long time.
This makes ultrasonic baths a poor choice for many applications. The probe vibrates rapidly, thereby transferring its ultrasonic energy to the sample.
Instead of energy being spread diffusely, the particles directly surrounding the probe get blasted with massive amounts of energy. Bubbles form and collapse in the surrounding solution, creating shear and shock waves. The highly localized intensity of the probe means the sonication process is more efficient and effective than in a bath. This makes ultrasonic homogenization ideal for particle size reduction and cell disruption.
Small particle sizes can be achieved with short processing times. Applications include breaking apart suspended cells, emulsification, and dispersion of nanoparticles. The most common method is to freeze and grind the sample using liquid nitrogen. It can also be done using a non-binding abrasive.
This method is effective for virtually all tissue types including seeds. Gentle Cell Disruption Techniques Gentle disruption consists of freeze-thaw lysis, enzymatic lysis, detergent lysis and osmotic lysis. Freeze-thaw lysis is pretty much as it sounds, using liquid nitrogen or a freezer to freeze the cells and then allow them to thaw. When cells are frozen the water inside the cells expands as it freezes causing the cells to burst open.
This method is effective for mammalian cells. Enzymatic lysis consists of suspending the cells in iso-osmotic buffers containing enzymes that digest the cell wall ie. Zymolyase for yeast cells, and lysozyme for bacterial cells. This lysis method is often used in conjunction with another disruption technique usually sonication to ensure complete lysis of the sample. This technique is effective with bacteria, yeast, fungi, algae, non-seed plant material and mammalian cell culture.
Detergent lysis involves suspending the cells in a detergent solution to solubilize the cell membrane, releasing the cell contents.
0コメント